Search results for " Osteoblast"

showing 8 items of 8 documents

Cabozantinib targets bone microenvironment modulating human osteoclast and osteoblast functions

2016

Cabozantinib, a c-MET and vascular endothelial growth factor receptor 2 inhibitor, demonstrated to prolong progression free survival and improve skeletal disease-related endpoints in castration-resistant prostate cancer and in metastatic renal carcinoma. Our purpose is to investigate the direct effect of cabozantinib on bone microenvironment using a total human model of primary osteoclasts and osteoblasts.Osteoclasts were differentiated from monocytes isolated from healthy donors; osteoblasts were derived from human mesenchymal stem cells obtained from bone fragments of orthopedic surgery patients. Osteoclast activity was evaluated by tartrate resistant acid phosphatase (TRAP) staining and …

0301 basic medicinePyridines -- pharmacologyPyridinesPyridineImmunoenzyme TechniqueOsteoclastsApoptosisRANK Ligand -- genetics -- metabolismImmunoenzyme Techniqueschemistry.chemical_compoundBone Resorption -- drug therapy -- metabolism -- pathology0302 clinical medicineOsteogenesisCathepsin KMedicineAnilidesAnilides -- pharmacologyOsteoprotegerin -- genetics -- metabolismOsteoclasts -- cytology -- drug effects -- physiologyHuman primary cellCells CulturedTartrate-resistant acid phosphataseReceptor Activator of Nuclear Factor-kappa B -- genetics -- metabolismbiologyProto-Oncogene Proteins c-met -- genetics -- metabolismReceptor Activator of Nuclear Factor-kappa BReverse Transcriptase Polymerase Chain ReactionOsteoblastOsteogenesiOsteoblastCell DifferentiationSciences bio-médicales et agricolesProto-Oncogene Proteins c-metOsteoblasts -- cytology -- drug effects -- physiologymedicine.anatomical_structureCell Differentiation -- drug effectsOncologyRANKL030220 oncology & carcinogenesishuman primary cellsOsteoclastosteoprotegerin (OPG)bone microenvironmentHumanResearch Papermusculoskeletal diseasesmedicine.medical_specialtyCabozantinibBlotting WesternOsteogenesis -- drug effects -- physiologyReal-Time Polymerase Chain ReactionBone resorption03 medical and health sciencesOsteoprotegerinOsteoclastcabozantinibInternal medicineHumansRNA MessengerBone ResorptionCell ProliferationOsteoblastsbusiness.industryRANK LigandAnilideOsteoprotegerinApoptosiBone microenvironment; Cabozantinib; Human primary cells; Osteoprotegerin (OPG); Receptor activator of nuclear factor-kb ligand (RANKL); Anilides; Apoptosis; Blotting Western; Bone Resorption; Cell Differentiation; Cell Proliferation; Cells Cultured; Humans; Immunoenzyme Techniques; Osteoblasts; Osteoclasts; Osteogenesis; Osteoprotegerin; Proto-Oncogene Proteins c-met; Pyridines; RANK Ligand; RNA Messenger; Real-Time Polymerase Chain Reaction; Receptor Activator of Nuclear Factor-kappa B; Reverse Transcriptase Polymerase Chain Reaction; Oncology030104 developmental biologyEndocrinologychemistrybiology.proteinbusinessRNA Messenger -- geneticsreceptor activator of nuclear factor-kb ligand (RANKL)

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Mesenchymal stromal cells and rheumatic diseases: new tools from pathogenesis to regenerative therapies

2015

In recent years, mesenchymal stromal cells (MSCs) have been largely investigated and tested as a new therapeutic tool for several clinical applications, including the treatment of different rheumatic diseases. MSCs are responsible for the normal turnover and maintenance of adult mesenchymal tissues as the result of their multipotent differentiation abilities and their secretion of a variety of cytokines and growth factors. Although initially derived from bone marrow, MSCs are present in many different tissues such as many peri-articular tissues. MSCs may exert immune-modulatory properties, modulating different immune cells in both in vitro and in vivo models, and they are considered immune-…

AdultCancer ResearchpathogenesiCellular differentiationImmunologyCell- and Tissue-Based TherapyBone Marrow CellsMesenchymal Stem Cell TransplantationRegenerative MedicineRegenerative medicineAutoimmune DiseaseAutoimmune DiseasesChondrocytesImmune systemIn vivoBone MarrowRheumatic DiseasesmedicineHumansImmunology and Allergyrheumatic diseaseGenetics (clinical)TransplantationOsteoblastsMesenchymal Stromal Cellbusiness.industryOsteoblastMesenchymal stem cellMesenchymal Stem CellsCell DifferentiationCell BiologyChondrocyteClinical trialmedicine.anatomical_structureregenerative therapyOncologymesenchymal stromal cells; pathogenesis; regenerative therapy; rheumatic disease; Adult; Autoimmune Diseases; Bone Marrow; Bone Marrow Cells; Cell Differentiation; Cell- and Tissue-Based Therapy; Chondrocytes; Humans; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Osteoblasts; Regenerative Medicine; Rheumatic DiseasesImmunologyBone Marrow CellBone marrowStem cellbusinessHuman

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miR-29b negatively regulates human osteoclastic cell differentiation and function: Implications for the treatment of multiple myeloma-related bone di…

2013

Skeletal homeostasis relies upon a fine tuning of osteoclast (OCLs)-mediated bone resorption and osteoblast (OBLs)-dependent bone formation. This balance is unsettled by multiple myeloma (MM) cells, which impair OBL function and stimulate OCLs to generate lytic lesions. Emerging experimental evidence is disclosing a key regulatory role of microRNAs (miRNAs) in the regulation of bone homeostasis suggesting the miRNA network as potential novel target for the treatment of MM-related bone disease. Here, we report that miR-29b expression decreases progressively during human OCL differentiation in vitro. We found that lentiviral transduction of miR-29b into OCLs, even in the presence of MM cells,…

Bone diseasePhysiologyCellular differentiationCathepsin KClinical BiochemistryGene ExpressionOsteoclastsOsteolysisMMP9Cathepsin KCells CulturedTartrate-resistant acid phosphataseTumorCulturedReceptor Activator of Nuclear Factor-kappa BGenes fosCell DifferentiationOsteoblastCell biologyIsoenzymesmultiple myelomamedicine.anatomical_structureMatrix Metalloproteinase 9osteoclastMatrix Metalloproteinase 2medicine.medical_specialtyfosCellsAcid PhosphataseBiologyCollagen Type IBone resorptionCell LineOsteoclastCell Line TumorInternal medicinemedicineHumansBone ResorptionOsteoblastsmicroRNA.NFATC Transcription FactorsTartrate-Resistant Acid PhosphatasemiR-29bCell Biologymedicine.diseaseActinsMicroRNAsEndocrinologyGenesAcid Phosphatase; Actins; Bone Resorption; Cathepsin K; Cell Differentiation; Cell Line Tumor; Cells Cultured; Collagen Type I; Gene Expression; Genes fos; Humans; Isoenzymes; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; MicroRNAs; Multiple Myeloma; NFATC Transcription Factors; Osteoblasts; Osteoclasts; Osteolysis; Receptor Activator of Nuclear Factor-kappa BJournal of Cellular Physiology

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Isolation and characterization of a murine resident liver stem cell.

2008

Increasing evidence provides support that mammalian liver contains stem/progenitor cells, but their molecular phenotype, embryological derivation, biology and their role in liver cell turnover and regeneration remain to be further clarified. In this study, we report the isolation, characterization and reproducible establishment in line of a resident liver stem cell (RLSC) with immunophenotype and differentiative potentiality distinct from other previously described liver precursor/stem cells. RLSCs, derived from fetal and neonatal murine livers as well as from immortalized hepatocytic MMH lines and established in lines, are Sca+, CD34-, CD45-, alpha-fetoprotein+ and albumin-. This molecular…

Cellular differentiationLiver Stem CellCell SeparationBiologyImmunophenotypingLiver progenitor cellsMiceChondrocyteshepatocyteAnimalsCell LineageProgenitor cellLiver progenitor cells; hepatocyte; differentiationMolecular BiologyCells CulturedMultipotent Stem CellOligonucleotide Array Sequence AnalysisNeuronsOsteoblastsAnimalOligonucleotide Array Sequence AnalysiLiver cellOsteoblastGene Expression ProfilingMultipotent Stem CellsMesenchymal stem cellCell DifferentiationCell BiologydifferentiationNeuronChondrocyteMolecular biologyLiver regenerationCell biologyPhenotypeAnimals NewbornLiverMultipotent Stem CellHepatocytesStem cellAnimals; Animals Newborn; Cell Differentiation; Cell Lineage; Cell Separation; Cells Cultured; Chondrocytes; Gene Expression Profiling; Hepatocytes; Immunophenotyping; Liver; Mice; Multipotent Stem Cells; Neurons; Oligonucleotide Array Sequence Analysis; Osteoblasts; Phenotype; Molecular Biology; Cell BiologyCell death and differentiation

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Gallium modulates osteoclastic bone resorption in vitro without affecting osteoblasts.

2010

Gallium (Ga) has been shown to be effective in the treatment of disorders associated with accelerated bone loss, including cancer-related hypercalcemia and Paget's disease. These clinical applications suggest that Ga could reduce bone resorption. However, few studies have studied the effects of Ga on osteoclastic resorption. Here, we have explored the effects of Ga on bone cells in vitro.In different osteoclastic models [osteoclasts isolated from long bones of neonatal rabbits (RBC), murine RAW 264.7 cells and human CD14-positive cells], we have performed resorption activity tests, staining for tartrate resistant acid phosphatase (TRAP), real-time polymerase chain reaction analysis, viabili…

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Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures

2019

In this paper, the effect of femtosecond laser nanotexturing of surfaces of Ti6Al4V and Zr implants on their biological compatibility is presented and discussed. Highly regular and homogeneous nanostructures with sub-micrometer period were imprinted on implant surfaces. Surfaces were morphologically and chemically investigated by SEM and XPS. HDFa cell lines were used for toxicity and cell viability tests, and subcutaneous implantation was applied to characterize tissue response. HDFa proliferation and in vivo experiments evidenced the strong influence of the surface topography compared to the effect of the surface elemental composition (metal or alloy). The effect of protein adsorption fro…

Materials scienceNanostructureSurface PropertiesBiomedical EngineeringPharmaceutical ScienceMedicine (miscellaneous)chemistry.chemical_elementBioengineering02 engineering and technologyOsseointegrationCell proliferation; Femtosecond laser; Fluorescence; HR-LIPSS; Nanotexturing; Cell Differentiation; Humans; Lasers; Nanostructures; Osseointegration; Osteoblasts; Osteogenesis; Surface Properties; Titanium; Zirconium; Prostheses and ImplantsFluorescencelaw.invention03 medical and health sciencesX-ray photoelectron spectroscopylawOsseointegrationOsteogenesisAlloysHumansGeneral Materials ScienceCell proliferation030304 developmental biologyTitanium0303 health sciencesOsteoblastsLasersTitanium alloyCell DifferentiationProstheses and Implants021001 nanoscience & nanotechnologyLaserNanotexturingNanostructuresFemtosecond laserchemistryFemtosecondBiophysicsMolecular MedicineHR-LIPSSZirconiumNanotexturingCell proliferationFluorescenceHR-LIPSSFemtosecond laser0210 nano-technologyTitaniumProtein adsorption

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IL RANK LIGANDO QUALE TARGET TERAPEUTICO NEL TRATTAMENTO DELLE PAZIENTI ANZIANE CON OSTEOPOROSI

2013

Cortical and trabecular bone undergo a continuous and balanced remodeling process, which consists of a phase of resorption, mediated by osteoclasts, and a phase of neoformation mediated by osteoblasts. The activity of both, osteoblasts and osteoclasts, is regulated by the osteocytes, which are the most abundant cells in bone tissue. An imbalance of this process with an excessive resorption activity causes a loss of bone mass and microarchitectural deterioration of the skeleton, with consequent reduction of bone strength and increased risk of fractures. The ligand for the “Receptor Activator of Nuclear Factor Kappa- B” (RANK-L), a protein expressed by osteoblasts, plays a fundamental role in…

Settore MED/09 - Medicina InternaBone remodeling RANK RANKL Denosumab Osteoblast Osteoclast Osteocyte

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Antitumor Effect of Cabozantinib in Bone Metastatic Models of Renal Cell Carcinoma

2021

Background: The presence of bone metastases in renal cell carcinoma (RCC) negatively affects patients’ survival. Data from clinical trials has highlighted a significant benefit of cabozantinib in bone metastatic RCC patients. Here, we evaluated the antitumor effect of cabozantinib in coculture models of renal cell carcinoma (RCC) and osteoblasts (OBs) to investigate whether and how its antiproliferative activity is influenced by OBs. Methods: Bone/RCC models were generated, coculturing green fluorescent protein (GFP)-tagged Caki-1 and 786-O cells with human primary OBs in a “cell–cell contact” system. RCC proliferation and the OB molecular profile were evaluated after the cabozantinib treat…

renal cell carcinomaCabozantinibQH301-705.5Biologyurologic and male genital diseasesGeneral Biochemistry Genetics and Molecular BiologyArticleGreen fluorescent proteinchemistry.chemical_compoundCabozantinib; Osteoblasts; Renal cell carcinomaRenal cell carcinomacabozantinibmedicineBiology (General)Antitumor activityGeneral Immunology and MicrobiologyCell growthOsteoblastosteoblastsmedicine.diseasefemale genital diseases and pregnancy complicationsInhibitory potencychemistryCancer researchMolecular ProfileGeneral Agricultural and Biological SciencesBiology

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